anti cd3 cd28 antibodies (Bio X Cell)

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Bio X Cell anti cd3 cd28 antibodies
Anti Cd3 Cd28 Antibodies, supplied by Bio X Cell, used in various techniques. Bioz Stars score: 97/100, based on 357 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/invivomab+anti-mouse+cd28/InVivoMAb+anti-mouse+CD28/pmc12963920-413-4-12
Average 97 stars, based on 357 article reviews

anti cd3 cd28 antibodies - by Bioz Stars, 2026-09

97/100 stars

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Cell Culture:

Article Title: Targeting tumor-intrinsic CDK1/Cyclin B1 complex improves responses to immunotherapy in pancreatic cancer.
Article Snippet: Immunotherapies, especially immune checkpoint blockade, has achieved considerable success in multiple cancers; however, patients with “cold tumors”, in particular pancreatic cancer, rarely respond, largely due to insufficient T cell activation and infiltration within the tumor microenvironment (TME).. To understand the mechanisms whereby tumor cells regulate T cell activation, we conducted an in vivo CRISPR-Cas9 screen, and identified the CDK1/Cyclin B1 complex as a previously unrecognized tumor-intrinsic driver of immune evasion in pancreatic cancer.. Genetic ablation or pharmacological inhibition of the CDK1/Cyclin B1 complex induced a T cell-inflamed TME, which synergized with PD-1 blockade to suppress tumor growth.

Article Title: Sulfur partitioning from cysteine controls T cell proliferation and effector function.
Article Snippet: .. CD8+ T cells were activated using plate-bound anti-CD3 (5 μg/ml; InVivoMab antimouse CD3, BioXCell, BE0002) and soluble anti-CD28 (0.5 μg/ml; InVivoMab anti-mouse CD28, BioXCell, BE0015) in T cell culture medium (TCM): RPMI 1640 media (Invitrogen) supplemented with 10% fetal calf serum (Gibco), 4 mM L-glutamine, 100 U/ml penicillin-streptomycin, 100 U/ml IL-2 (PeproTech), 55 μM beta-mercaptoethanol in a humidified incubator at 37◦C, atmospheric oxygen supplemented with 5% CO2. ..

Labeling:

Article Title: Maternal Krüppel-like factor 2 (KLF2)+ CD4 T cells promote fertility and fetal tolerance.
Article Snippet: For evaluating suppressive function, CD4 cells from the spleen and peripheral lymph nodes of KLF2(GFP) FOXP3(RFP) dual reporter mice were initially purified by negative selection (Miltenyi, #130- 104- 454), stained with viability dye, anti- CD4, and anti- CD44 antibodies, followed by sorting (BD S6). .. Thereafter, suppression was assayed by coculture with 105 cell trace violet- labeled (5 mM for 20 min at 37 °C) responder splenocytes from congenially discordant (CD45.1) mice on the C57BL/6 background, and stimulation with anti- CD3 (2.5 mg/mL, clone 145- 2C11, BioXcell) and anti- CD28 (1.25 mg/mL, clone 37.51, BioXcell) in 200 μL DMEM (supplemented with 10% fetal bovine serum, 1% L- glutamine, 10 mM HEPES, 1% penicillin- streptomycin) at 37 °C with 5% CO2 for 3 d as described (35). ..

FACS:

Article Title: The Glucose Transporter GLUT3 Controls Regulatory T Cell Function
Article Snippet: For iTreg differentiation, CD4 + T cells were cultured with 2.5 μg/mL anti-IL-4 (clone 11B11), 2.5 μg/mL anti-IFN-γ (clone XMG 1.2; both Bio X Cell), 10 ng/mL human IL-2, and 5 ng/mL recombinant human TGF-β1 (both PeproTech). .. FACS-sorted Treg cells were activated on Delta surface 96-well plates (Nunc) with 0.25 ng/mL anti-CD3 (clone 145-2C1) and 1 ng/mL anti-CD28 (clone 37.51; both Bio X Cell), 20 ng/mL recombinant human IL2 and 10 ng/mL murine IL7 for three days at a density of 2×10 6 cells/mL. .. Mitochondrial respiration and glycolytic activity of Treg cells were assessed by measuring oxygen consumption rate (OCR) and glycolytic proton efflux rate (PER), respectively, using an XFe96 extracellular flux analyzer (Seahorse Bioscience) as previously described ( ).

Recombinant:

Isolation:

Article Title: Inflammatory arthritis irAE may represent a unique autoimmune disease primarily driven by T cells but likely not autoantibodies.
Article Snippet: B cells were labeled with CellTrace Violet (Invitrogen) and cultured in RPMI 1640 medium supplemented with 10% (v/v) FBS and 1% penicillin- streptomycin. .. A total of 0.5 million isolated CD4+ T cells or CD8+ T cells was stimulated with plate- coated anti- CD3 (10 μg/ml; BioXCell, catalog no. BE0001- 2) and anti- CD28 (BioXCell, catalog no. BE0291), rhIL- 6 (100 ng/ml), rhIFN- α2 (100 ng/ ml), rhIL- 12 (100 ng/ml), or the combination of rhIL- 6, rhIFN- α2, and rhIL- 12 for 5 days. .. Condition 1: LPS (3 μg/ml; Sigma- Aldrich, catalog no. L288025MG) and recombinant mouse IL- 4 (10 ng/ml; R&D Systems, catalog no. 404- ML- 100/CF) plus recombinant human BAFF (20 ng/ ml; BioLegend, catalog no. 7449534).

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